Part:BBa_K1391019:Experience

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Applications of BBa_K1391019

MAV1212_hEF1a_L7Ae is a expression vector. When transfected, it constitutively expresses L7Ae, a translational repressor. MAV1212_hEF1a_L7Ae is intended to be used as an inverter for the presence of specific miRNAs.

LacZ bordered by Golden Gate Assembly sites follows L7Ae. LacZ can be replaced with miR target sites, which will lead to L7Ae being repressed by the presence of miRNAs.

For example: MAV1212-hEF1a-L7Ae-miR, coupled with pEXPR_2xk-turn_hEF1a_eBFP2, is a sensor that expresses eBFP2 in the presence of high concentrations of miR.

User Reviews

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